Before and after results from biotin injections depend entirely on whether you have actual biotin deficiency or are taking them without deficiency for cosmetic reasons. For people with confirmed biotin deficiency, before biotin treatment they typically have symptoms including significant hair loss or thinning that can be quite dramatic; skin rashes, dermatitis or scaly skin around eyes, nose and mouth; very brittle, splitting or cracking nails; possible neurological symptoms like numbness, tingling or depression; fatigue or lethargy; and in severe cases, developmental delays in children. After biotin injection treatment for confirmed deficiency, assessed over weeks to months as biotin levels normalize, people experience dramatic improvement in hair loss slowing or stopping with gradual regrowth beginning; skin rashes healing and skin quality normalizing; nails becoming stronger and healthier; neurological symptoms improving; energy levels returning to normal; and overall resolution of deficiency-related problems. These before and after improvements can be truly dramatic and life-changing for people with genuine biotin deficiency. However, for people without biotin deficiency taking injections for wellness or cosmetic purposes, the before and after picture is very different. Before injections, these people typically have normal biotin levels; may have hair thinning, brittle nails or skin concerns from other causes unrelated to biotin; and are seeking enhancement based on marketing claims. After biotin injections for people without deficiency, the realistic outcomes are minimal to no visible change in hair growth, thickness or quality; little to no improvement in nail strength or growth; no significant change in skin quality or appearance; possible placebo effect making them feel their hair or nails are better; and essentially unchanged appearance compared to baseline. Any improvements reported are more likely due to placebo effect, natural hair growth cycles that would occur anyway, other dietary or lifestyle changes, resolution of other nutritional deficiencies, or simply the passage of time rather than biotin specifically. The wellness industry often shows dramatic before and after photos for biotin treatments, but these are typically misleading because they may show people with actual deficiency (rare) and attribute improvement solely to biotin; they use other treatments alongside biotin without disclosure; photos are taken at different stages of natural hair growth cycles; lighting, styling and photo manipulation create the appearance of improvement; or they are simply not genuine and are marketing materials rather than documented clinical results. It is crucial to understand that dramatic before and after transformations from biotin are only realistic for people with confirmed biotin deficiency, which is extremely rare. For the vast majority of people with normal biotin levels, before and after appearance will be essentially unchanged regardless of biotin supplementation. If you are considering biotin injections hoping for visible improvement in hair, nails or skin, realistic expectations are that without confirmed deficiency, you will likely see no meaningful change; any perceived improvement is more likely placebo effect than actual biotin benefit; and you should address the actual underlying causes of your concerns rather than pursuing unproven biotin supplementation. For genuine hair loss concerns, evidence-based treatments like minoxidil, finasteride (for men), or other appropriate interventions based on proper diagnosis are more effective than biotin supplementation. For nail concerns, identifying and treating underlying causes (nutritional deficiencies, thyroid issues, fungal infections, etc.) is more productive than biotin supplementation in non-deficient individuals. Most dermatologists and trichologists will tell you that biotin supplementation for cosmetic purposes in people without deficiency rarely produces the dramatic before and after results that marketing suggests, and that spending money and time on biotin injections without proper diagnosis is generally not worthwhile. If you have concerns about hair loss, nail health or skin quality, proper medical evaluation to identify the actual cause is far more valuable than speculative biotin supplementation, allowing you to pursue treatments with proven effectiveness for your specific condition rather than relying on a supplement that is only beneficial for the rare cases of true biotin deficiency.